This function allows to subset the mif object into compartments. For instance a mif object includes all cells and the desired analysis is based on only the tumor or stroma compartment then this function will subset the spatial list to just the cells in the desired compartment
subset_mif(mif, classifier, level, markers)An MIF object
Column name for spatial dataframe to subset
Determines which level of the classifier to keep.
vector of
mif object where the spatial list only as the cell that are the specified level.
#' #Create mif object
library(dplyr)
x <- create_mif(clinical_data = example_clinical %>%
mutate(deidentified_id = as.character(deidentified_id)),
sample_data = example_summary %>%
mutate(deidentified_id = as.character(deidentified_id)),
spatial_list = example_spatial,
patient_id = "deidentified_id",
sample_id = "deidentified_sample")
markers = c("CD3..Opal.570..Positive","CD8..Opal.520..Positive",
"FOXP3..Opal.620..Positive","PDL1..Opal.540..Positive",
"PD1..Opal.650..Positive","CD3..CD8.","CD3..FOXP3.")
mif_tumor = subset_mif(mif = x, classifier = 'Classifier.Label',
level = 'Tumor', markers = markers)