All functions

STclust()

STclust: Detect clusters of spots/cells

STdiff()

STdiff: Differential gene expression analysis for spatial transcriptomics data

STdiff_volcano()

STdiff_volcano: Generates volcano plots from STdiff results

STenrich()

STenrich

STgradient()

STgradient: Tests of gene expression spatial gradients

SThet()

SThet: Computes global spatial autocorrelation statistics on gene expression

STlist-class

Definition of an STlist object class.

STlist()

STlist: Creation of STlist objects for spatial transcriptomics analysis

STlist_to_disk()

STlist_to_disk: Move an STlist's count matrices to on-disk parquet

STlist_to_memory()

STlist_to_memory: Bring an on-disk STlist back into memory

STniche()

STniche (mclust version): Detect functional spatial domains with model-based clustering

STplot()

STplot: Plots of gene expression, cluster memberships, and metadata in spatial context

STplot_interpolation()

STplot_interpolation: Visualize gene expression surfaces

as_Seurat()

as_Seurat: Convert an STlist to a Seurat object

compare_SThet()

compare_SThet: Compares spatial autocorrelation statistics across samples

dim(<STlist>)

dim: Prints the dimensions of count arrays within an STList object.

distribution_plots()

per_unit_counts: Generates distribution plots of spot/cell meta data or gene expression

filter_data()

filter_data: Filters cells/spots, genes, or samples

gene_interpolation()

gene_interpolation: Spatial interpolation of gene expression

get_gene_meta()

get_gene_meta: Extract gene-level metadata and statistics

is_backed()

is_backed: Is an STlist's expression data stored on disk?

load_images()

load_images: Place tissue images within STlist

plot_counts()

plot_counts: Generates plots for the distribution of counts

plot_image()

plot_image: Generate a ggplot object of the tissue image

plot_stniche()

Plot STniche results

pseudobulk_dim_plot()

pseudobulk_dim_plot: Plot PCA of pseudobulk samples

pseudobulk_heatmap()

pseudobulk_heatmap: Heatmap of pseudobulk samples

pseudobulk_samples()

pseudobulk_samples: Aggregates counts into "pseudo bulk" samples

show(<STlist>)

show: Prints overview of STList oject.

spatial_metadata()

spatial_metadata: Prints the names of the available spot/cell annotations

summarize_STlist()

summarize_STlist: Generates a data frame with summary statistics

summary(<STlist>)

summary: Prints overview of STList oject.

tissue_names()

tissue_names: Prints the names of the tissue samples in the STlist

transform_data()

transform_data: Transformation of spatial transcriptomics data

validate_store()

validate_store: Check an on-disk STlist against its manifests